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Himeras were expressed with either a Roc1a- or Roc1b-grf (genomic rescue fragment). The Roc1a-grf was previously described [24] and contains 980 bp upstream of the Start codon and 620 bpBinding Determinants of Rocsdownstream of the stop codon. A FLAG- or V5- tag was inserted in frame immediately downstream of the initiating methionine. The Roc1b-grf, containing 840 bp upstream from the Start codon

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